In among all the brands of DNA polymerase, which one stand out among all?
I found out Bioline's MyTaq look pretty good when compare to other vendors' DNA polymerase with DNA template of 61%GC.
Anyone uses Bioline's product before?
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5 replies to this topic
#2
Curtis
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Posted 09 January 2013 - 07:27 AM
you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment
#3
hobglobin
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Posted 09 January 2013 - 08:43 AM
I guess it depends on the target DNA in the end and you have to try out...there is no best one.
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#4
badguy
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Posted 10 January 2013 - 06:49 PM
What Bioline's polymerase you used?you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment
#5
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#6
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Posted 09 January 2015 - 11:50 AM
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