Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

DNA polymerase for GC rich template?

taq

  • Please log in to reply
5 replies to this topic

#1 badguy

badguy

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 49 posts
2
Neutral

Posted 08 January 2013 - 11:55 PM

In among all the brands of DNA polymerase, which one stand out among all?
I found out Bioline's MyTaq look pretty good when compare to other vendors' DNA polymerase with DNA template of 61%GC.
Anyone uses Bioline's product before?

#2 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,201 posts
63
Excellent

Posted 09 January 2013 - 07:27 AM

you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment

#3 hobglobin

hobglobin

    Growing old is mandatory, growing up is optional...

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,615 posts
127
Excellent

Posted 09 January 2013 - 08:43 AM

I guess it depends on the target DNA in the end and you have to try out...there is no best one.

A single lie is reproachable; a million lies is a statistic.
D. J. T.


#4 badguy

badguy

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 49 posts
2
Neutral

Posted 10 January 2013 - 06:49 PM

you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment

What Bioline's polymerase you used?

#5 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,201 posts
63
Excellent

Posted 10 January 2013 - 07:38 PM

BIO-X-ACT™ Long DNA Polymerase

#6 earthox

earthox

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 09 January 2015 - 11:50 AM

www.earthox.net 







Also tagged with one or more of these keywords: taq

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.