Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

0

DNA polymerase for GC rich template?

Started by badguy, Jan 08 2013 11:55 PM

taq

Please log in to reply

4 replies to this topic

#1 badguy

Enthusiast

Active Members
47 posts

2
Neutral

Posted 08 January 2013 - 11:55 PM

In among all the brands of DNA polymerase, which one stand out among all?
I found out Bioline's MyTaq look pretty good when compare to other vendors' DNA polymerase with DNA template of 61%GC.
Anyone uses Bioline's product before?

Back to top

#2 Curtis

Metaller Scientist

Global Moderators
981 posts

45
Excellent

Posted 09 January 2013 - 07:27 AM

you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment

Back to top

#3 hobglobin

Growing old is mandatory, growing up is optional...

Active Members
5,261 posts

60
Excellent

Posted 09 January 2013 - 08:43 AM

I guess it depends on the target DNA in the end and you have to try out...there is no best one.

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

Back to top

#4 badguy

Enthusiast

Active Members
47 posts

2
Neutral

Posted 10 January 2013 - 06:49 PM

Curtis, on 09 January 2013 - 07:27 AM, said:

you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment

What Bioline's polymerase you used?