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DNA polymerase for GC rich template?

taq

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4 replies to this topic

#1 badguy

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Posted 08 January 2013 - 11:55 PM

In among all the brands of DNA polymerase, which one stand out among all?
I found out Bioline's MyTaq look pretty good when compare to other vendors' DNA polymerase with DNA template of 61%GC.
Anyone uses Bioline's product before?

#2 Curtis

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Posted 09 January 2013 - 07:27 AM

you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment

#3 hobglobin

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Posted 09 January 2013 - 08:43 AM

I guess it depends on the target DNA in the end and you have to try out...there is no best one.
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#4 badguy

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Posted 10 January 2013 - 06:49 PM

you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment

What Bioline's polymerase you used?

#5 Curtis

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Posted 10 January 2013 - 07:38 PM

BIO-X-ACT™ Long DNA Polymerase





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