DNA polymerase for GC rich template?taq
Posted 08 January 2013 - 11:55 PM
I found out Bioline's MyTaq look pretty good when compare to other vendors' DNA polymerase with DNA template of 61%GC.
Anyone uses Bioline's product before?
Posted 09 January 2013 - 07:27 AM
Posted 09 January 2013 - 08:43 AM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 10 January 2013 - 06:49 PM
What Bioline's polymerase you used?
you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment