I red that methylated DNA has less tranformation efficiency than unmethylated. How can i get my plamid unmethylated?
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#2
bob1
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Posted 08 January 2013 - 06:27 PM
You can use strains of bugs without the methyl transferases. These strains will be Dam-/Dcm-. I don't know of any way to do this post extraction if that is what you are asking.
#3
Pangea
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Posted 09 January 2013 - 05:41 AM
Can you give some example like JM110 or others? Which strain is most frequently used in the Labs?
