Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Dam Dcm Negative Strain

Methylation

  • Please log in to reply
2 replies to this topic

#1 Pangea

Pangea

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 97 posts
7
Neutral

Posted 08 January 2013 - 01:14 PM

I red that methylated DNA has less tranformation efficiency than unmethylated. How can i get my plamid unmethylated?

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,619 posts
388
Excellent

Posted 08 January 2013 - 06:27 PM

You can use strains of bugs without the methyl transferases. These strains will be Dam-/Dcm-. I don't know of any way to do this post extraction if that is what you are asking.

#3 Pangea

Pangea

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 97 posts
7
Neutral

Posted 09 January 2013 - 05:41 AM

Can you give some example like JM110 or others? Which strain is most frequently used in the Labs?





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.