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collagen coating of transwell inserts


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#1 Mahtab

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Posted 08 January 2013 - 08:16 AM

Hello,

I seeded the Caco2 cells in transwell inserts to do a permeability assay in 21 days. I just forgot to suck off the collagen solution that I had used for coating, before I seed the cells. Will it make a big problem for me or I can still count on the cells? I don't know how seriously it affects the forming of cell monolayer and the permeability results.
Thanks in advance!

#2 Curtis

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Posted 08 January 2013 - 11:06 PM

I think just remove them, wash them and re-culture to get an even monolayer.

#3 Mahtab

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Posted 09 January 2013 - 07:45 AM

Thank you!




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