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Why there are blobs in my sheared DNA bands?

Sheared DNA

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#1 ChIP Kick

ChIP Kick

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Posted 07 January 2013 - 10:29 AM

Hi, I have some problems with my sheared DNA now. Hope you can help me! Attached are two images for the same DNA gel. Image were taken at different time. One is at 1:00 pm and another is at 3:11pm.

It seems like that I get some smeared DNA. But I do not know why there are blobs at the bottom of the band. I treated samples with proteinase K, incubate at 58 degree Celsius, overnight. Then did RNase A treatment at 37degree Celsius for 1 hour. After that, run a agarose gel at 53 voltage. Most sheared DNA bands in web don't have blobs. Could you give me some suggestions about that?

Thanks a lot!

Attached Thumbnails

  • 1pm gel.jpg
  • 3pm gel.jpg





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