5 replies to this topic

[emadsadeghian]

Hi people:
I recently found this website from openwetware:
http://openwetware.o.../Qiagen_Buffers
At the bottom of the page, it explains how we can actually re use the spin column. As you all know Qiagen doesnt sell columns separately and they come as very expensive kits.
My question was if this technique also the ingredients for other buffers are trustworthy and anyone has done it before?

thanks

[bob1]

In general the protocols on Openwetware are reliable, the labs that have provided the majority are some of the foremost in molecular biology.

The technique does work, but I wouldn't recommend using the recycled columns for different DNAs so as to eliminate potential sources of contamination of your extractions.

[neuron]

This is really good, I dint know that columns can be reused , at least we can save some and use them in emergency ..but I was wondering 1M Hcl is too strong acid, won't it disrupt or damage the membrane? or silica is stable even in acid???

[emadsadeghian]

Neuron, yes, i think 1M HCl is a strong acid and also the procedure is too long. my question was if anyone has re used the column and was it any contaminations due to re using of the columns ? Thanks

[hobglobin]

Some companies offer also kits to regenerate silica columns and universal buffer sets for refilling.
Biotechniques paper
Buffers
(just to be sure or if you are too lazy )

[neuron]

This is really interesting hobglobin .

emadsadeghian, on 08 January 2013 - 04:19 AM, said:

Neuron, yes, i think 1M HCl is a strong acid and also the procedure is too long. my question was if anyone has re used the column and was it any contaminations due to re using of the columns ? Thanks

I have no idea emadsadeghian, I have never done this, neither people do in my lab. We waste so many columns, reusing is a good idea. But yes we need to try first to see if we get any contamination. or like bob1 suggested we can reuse the same columns for same DNA in future.