Posted 06 January 2013 - 06:33 PM
I am working for BDNF measurement in human bloods.For this, I need to get my capture and detection Ab's working. My detection Ab is mouse monoclonal biotin conjugated. One-site ELISA worked for my biotin conjugated Mab. For last couple of weeks I have been optimizing the concentration of Mab's. My concentration for capture is 3ug/ml and for detection Ab is 2ug/ml. My problem is that, at lowest concentration of detection Ab, my blank is too high. I tried using different blocking buffers, washing buffer. My supervisor says that I dont have any problems with buffer. Does Acetic Acid interfere the biotin? Can you guys please help me out with the biotin conjugated detection Ab (mouse monoclonal) protocol?
Posted 08 January 2013 - 04:33 PM
Posted 22 January 2013 - 08:13 PM