Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Cisplatin affect of HeLa

  • Please log in to reply
1 reply to this topic

#1 Lim Cheng Hoe

Lim Cheng Hoe


  • Members
  • Pip
  • 1 posts

Posted 06 January 2013 - 12:25 AM


I have tested apoptotic activity for Cisplatin againts HeLa cells using RayBio® Human Apoptosis Antibody Array Kit.
The results is confusing where expression of p53 and caspase 3 decreases overtime but level of XiAP decreases dramatically.

So, does it shows apoptosis?

#2 doxorubicin



  • Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 194 posts

Posted 06 January 2013 - 08:30 AM

Cisplatin should increase p53 expression and decrease XIAP expression, while increasing the amount of cleaved caspase-3. Based on the Raybiotech website, it seems they're array is detecting the apoptosis-induced cleavage product of caspase-3: http://www.raybiotec...optosis-arrays/ although you should try to confirm that their antibody isn't detecting full-length and cleaved forms. I suspect you are studying "noise".

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.