1 reply to this topic

[Lim Cheng Hoe]

Hi,

I have tested apoptotic activity for Cisplatin againts HeLa cells using RayBio® Human Apoptosis Antibody Array Kit.
The results is confusing where expression of p53 and caspase 3 decreases overtime but level of XiAP decreases dramatically.

So, does it shows apoptosis?

[doxorubicin]

Cisplatin should increase p53 expression and decrease XIAP expression, while increasing the amount of cleaved caspase-3.  Based on the Raybiotech website, it seems they're array is detecting the apoptosis-induced cleavage product of caspase-3:  http://www.raybiotec...rays/  although you should try to confirm that their antibody isn't detecting full-length and cleaved forms.  I suspect you are studying "noise".