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Cisplatin affect of HeLa


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#1 Lim Cheng Hoe

Lim Cheng Hoe

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Posted 06 January 2013 - 12:25 AM

Hi,

I have tested apoptotic activity for Cisplatin againts HeLa cells using RayBio® Human Apoptosis Antibody Array Kit.
The results is confusing where expression of p53 and caspase 3 decreases overtime but level of XiAP decreases dramatically.

So, does it shows apoptosis?

#2 doxorubicin

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Posted 06 January 2013 - 08:30 AM

Cisplatin should increase p53 expression and decrease XIAP expression, while increasing the amount of cleaved caspase-3. Based on the Raybiotech website, it seems they're array is detecting the apoptosis-induced cleavage product of caspase-3: http://www.raybiotec...optosis-arrays/ although you should try to confirm that their antibody isn't detecting full-length and cleaved forms. I suspect you are studying "noise".




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