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Insulin production

insulin

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#1 Pangea

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Posted 05 January 2013 - 12:59 PM

What would be the best way to express and purify active insulin?

#2 Curtis

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Posted 08 January 2013 - 09:12 PM

I don't know how much info you already have, they do this by cloning the two isoforms and transforming into e.coli. Perhaps you can check this animation:

http://www.abpischoo...tion_allTopic=1

If you need more in-depth material you need to check the Diabetes Journal under American Diabetes Association.

#3 Pangea

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Posted 09 January 2013 - 05:46 AM

Cool source thaks for the hint.





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