What would be the best way to express and purify active insulin?
2 replies to this topic
#1
Posted 05 January 2013 - 12:59 PM
#2
Posted 08 January 2013 - 09:12 PM
I don't know how much info you already have, they do this by cloning the two isoforms and transforming into e.coli. Perhaps you can check this animation:
http://www.abpischoo...tion_allTopic=1
If you need more in-depth material you need to check the Diabetes Journal under American Diabetes Association.
http://www.abpischoo...tion_allTopic=1
If you need more in-depth material you need to check the Diabetes Journal under American Diabetes Association.
#3
Posted 09 January 2013 - 05:46 AM
Cool source thaks for the hint.
Also tagged with one or more of these keywords: insulin
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