What would be the best way to express and purify active insulin?
Submit your paper to J Biol Methods today!
2 replies to this topic
#2
Curtis
-
- Global Moderators
-
- 1,073 posts
Metaller Scientist
55
Excellent
Excellent
Posted 08 January 2013 - 09:12 PM
I don't know how much info you already have, they do this by cloning the two isoforms and transforming into e.coli. Perhaps you can check this animation:
http://www.abpischoo...tion_allTopic=1
If you need more in-depth material you need to check the Diabetes Journal under American Diabetes Association.
http://www.abpischoo...tion_allTopic=1
If you need more in-depth material you need to check the Diabetes Journal under American Diabetes Association.
- Pangea likes this
#3
Pangea
-
- Active Members
-
- 97 posts
Enthusiast
7
Neutral
Neutral
Posted 09 January 2013 - 05:46 AM
Cool source thaks for the hint.
Also tagged with one or more of these keywords: insulin
Protocols and Techniques Forums →
Molecular Cloning →
*** Human Insulin promoter sequence***Started by Labrock, 05 Sep 2012  Insulin, promoter, cloning, PCR
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Human insulin for cell culture?Started by ioannap, 04 Apr 2012 cell culture, growth supplements and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
3T3-L1 differenciation and maintenanceStarted by fredUNH, 08 Feb 2012 adipocyte differenc, cell culture and 3 more...
|
|
|
