Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Changing Genomic Regions in Host Cell

Rercombination

  • Please log in to reply
1 reply to this topic

#1 Pangea

Pangea

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 97 posts
7
Neutral

Posted 05 January 2013 - 01:46 AM

How can i best delete a gene region of my host cell.? And how long time will it take?

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,709 posts
398
Excellent

Posted 05 January 2013 - 02:46 PM

You can use a recombination event using a plasmid which contains sequence found on either side of the desired KO region. Usually these sequences will also flank a reporter gene of some sort to help with identification of cells which have recombined.

Clonal selection of cells will take months! Make sure you are very good at your cell culture before you start so that you know what you are looking for, and so that you can check on the health of the cells, and be able to determine if the cells are behaving the same as the parent line (important if you need controls!).




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.