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Replication of the Plasmid DNA

plasmid dna enzyme

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#1 Pangea

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Posted 05 January 2013 - 01:01 AM

My Question would be which enzyme do we need in replicating Plasmids in E.coli? Any idea.

#2 bob1

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Posted 05 January 2013 - 02:53 PM

This sounds suspiciously like a homework question... so here's a hint rather than a full answer:

There are two very obvious ones. Both to do with DNA.

#3 Pangea

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Posted 05 January 2013 - 03:25 PM

You mean the Replication machinary.

#4 bob1

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Posted 06 January 2013 - 02:00 PM

Exactly...Helicase and a DNA pol are at least required.

#5 Pangea

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Posted 07 January 2013 - 07:33 AM

Thats shouldnt be a big deal, isnt?

#6 bob1

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Posted 07 January 2013 - 02:37 PM

I don't know what you mean by a big deal, but if you were referring to my reluctance to give a full answer - I do that so as to make you think, it helps you learn and to become a better scientist...

#7 Pangea

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Posted 08 January 2013 - 07:24 AM

:)




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