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TRYPSIN

Trypsin cell culture pro

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4 replies to this topic

#1 Pangea

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Posted 04 January 2013 - 02:51 PM

How can i produce Cell culture Trypsin? Any Idea?

#2 leelee

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Posted 04 January 2013 - 06:08 PM

Why do you want to produce it? It is cheap to buy :)

#3 Pangea

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Posted 05 January 2013 - 12:52 AM

To make it more cheaper ;). Dou you have an idea? Maybe i will get the clone from IMAGE Consortium. Or get the trypsin from slaughterhouse.

#4 leelee

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Posted 05 January 2013 - 01:42 AM

Honestly, I think it is a bad idea. The risk to the quality of your experiments is high, and how much will you really save?

It will be a heck of a lot of work to get it to a purity sufficient for cell culture, as you don't want to risk any potential contamination. Not just by microorganisms, but by other proteins, enzymes, hormones, growth factors etc- all of which could cause you problems with your cell culture.

A wise person once told me the most expensive thing in the lab is your time. And, like I said, trypsin is SO cheap, it just isn't worth trying to cheap out even further.

If you really don't want to shell out for liquid, cell culture grade trypsin- then have you considered at least purchasing the powder form and making it up yourself that way?

#5 Pangea

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Posted 05 January 2013 - 02:13 AM

Thanks for your Advice.




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