I want generate adherent cells to suspension cells. I not mean trypsinyzing. Can anyone help?
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How to transform adherent cells to suspension (CHO cells)
Started by Pangea, Jan 04 2013 02:45 PM
adherent suspension cell
2 replies to this topic
#2
Tabaluga
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Posted 05 January 2013 - 03:13 PM
Some adherent cells grow in suspension when you use serum-free media. However, be aware that this can change the characteristics of the cells ! I also know that sigma at least has a serum-free medium for CHO suspension cells, but I think it's expensive and probably other serum-free media are also going to do the trick.
#3
Denny
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Posted 06 January 2013 - 10:07 AM
There is a wealth of good information on the web about adapting CHO adherent cells to suspension culture and serum free medium, search for "adapting CHO adherent cells to suspension". You can find information from Invitrogen/Gibco,Thermo Scientific Hyclone, and other suppliers of
SFM... about adaption methods as well.
SFM... about adaption methods as well.
Edited by Denny, 06 January 2013 - 10:08 AM.
