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Used all ChIP lysis buffer


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#1 ChIP Kick

ChIP Kick

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Posted 04 January 2013 - 12:51 PM

Hi, I tried many times but I still cannot get a good sonication condition for my exp. Now I used all my lysis buffer which I bought from Millipore(EZ-Magna ChIP™ A Chromatin Immunoprecipitation Kit). I checked their web but no recipe for it.There are so many different recipes for lysis buffer. I do not know which one is good for my exp.

So is there someone who could give me suggestions to make my own cell lysis buffer and nuclear lysis buffer?

Thanks a lot!




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