Hi, I tried many times but I still cannot get a good sonication condition for my exp. Now I used all my lysis buffer which I bought from Millipore(EZ-Magna ChIP™ A Chromatin Immunoprecipitation Kit). I checked their web but no recipe for it.There are so many different recipes for lysis buffer. I do not know which one is good for my exp.
So is there someone who could give me suggestions to make my own cell lysis buffer and nuclear lysis buffer?
Thanks a lot!
Used all ChIP lysis buffer
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