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DNA extraction - PCR Problem


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#1 Hamidrad

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Posted 04 January 2013 - 10:52 AM

Hi all,
At first I should say that I dont know enough about PCR but I am starting to work with it in near time. I am a food technologist and now I am trying to extract dna from pulp and peel of banana. But the problem is here. One of my friend said to me that I'll get in trouble with ploidity of banana's dna (diploid or triploid ...). So let me know something about it.
Thanks a lot
Hamid

#2 Pangea

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Posted 04 January 2013 - 12:52 PM

You must have a isolation protocol, as far i know if you have good primers ploidity should not be a problem. You are not the first person working with. It tells you just the copy number of identical chromosomes. Isolated DNA should be clean.

#3 Hamidrad

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Posted 05 January 2013 - 02:23 AM

You must have a isolation protocol, as far i know if you have good primers ploidity should not be a problem. You are not the first person working with. It tells you just the copy number of identical chromosomes. Isolated DNA should be clean.

Thanks. But I didnt find survey on dna isolating from pulp and peel of bananas!! But there are a lot of research on dna extracting from leaf and root of it. So We should try to have good isolation and then select a suitable primers for pcr and dont focus on searching ploidity of banana's dna before dna extracting?... OK

#4 vilperte

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Posted 11 February 2013 - 03:15 AM

You should start with CTAB or SDS methods as a starting point. They are the most common methods and they are suitable for a huge range of species.

You could also try some DNA kit, such as DNeasy (QIAGEN), NucleoSpin Plant (Macherey-Nagel) and so on.




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