my competent cell grow on any agar plate
Posted 04 January 2013 - 04:50 AM
Since I have kept my original stock of cells before making them competent, I did the same test on the original stack and i saw colonies only on the tetracyclin plates (XL1 Blue is resistant to Tet). This means the procedure of producing the competent cell has somehow made my Ecoli strand a multiresistant strain.
My question is if this is possible and if not why this has happened to my batch?
Posted 04 January 2013 - 05:27 AM
Posted 04 January 2013 - 06:52 AM
Phage434, thanks for letting me know the source of this problem. probably ice or water contained these resistant organism
Edited by emadsadeghian, 04 January 2013 - 06:53 AM.
Posted 05 January 2013 - 03:01 PM
Posted 07 January 2013 - 06:28 AM
i carried out the experimental procedure again to make new competent cell from new stock of xl1 blue. I tested them with right antibiotics. They only grew on Tet and control (o antibiotic), It is great news. Its because I autoclaved my equipments before use and I made sure the whole procedure is absolute sterile eliminating the contamination problems.
Posted 07 January 2013 - 07:32 AM
Posted 08 January 2013 - 04:25 AM
Therefore i did not do any transformation. I just did normal testing on the cells