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Primers to amplify Kan operon from pet28

pet28 kanamycin

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2 replies to this topic

#1 nishachara

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Posted 04 January 2013 - 03:54 AM

Hi
I want to amplify kanamaycin drug cassette from pet 28 vector, is there any one knows the primer for this? since in the map they have not shown the RBS binding sites and promoters for the drug marker.
thanks in advance.
shashi

#2 Curtis

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Posted 08 January 2013 - 10:02 PM

go to NCBI and search for this vector, and get the full sequence of it. It must be there. Then find the Kan location according to the map.

Kan coding sequence 3995-4807

#3 neuron

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Posted 08 January 2013 - 11:33 PM

I just typed pet28 vector sequence on google and got this link-

http://sil.biochem.u...ET-28a_seq.html





Also tagged with one or more of these keywords: pet28, kanamycin

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