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Dox induction and cell density

NT2 cells Doxycycline

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#1 chabraha

chabraha

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Posted 03 January 2013 - 12:15 PM

I have a stably transduced NT2D1 cell population containing a Doxycycline inducible shRNA cassette (pTRIPZ). Normally these cells prefer to grow at a high density, but I think I've noticed that the Dox induction isn't as effective at higher cell densities..................anyone seen/heard similar effects?
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