5 replies to this topic

[Pangea]

How can İ prevent acylation (Lys) of my recombinant protein in E. coli or Mammalian cells?

[HOYAJM]

Could you treat the cells with an acyltransferase inhibitor? They are available commericially.

[Pangea]

This option also came in my mind but to knockout the gene would be Lethal i guess. In mammalian maybe one could use stable cell lines transfected with shRNA, or?

[Pangea]

Is there a way to screen a inhibitor collection?

[HOYAJM]

Im not quite following. You fear that if you use an acyltransferase inhibitor you will "knock out" the gene/protein function? If you want to prevent acylation, these inhibitors seem like the logical choice. It will inhibit acylation of all proteins, but there will be a recommended dose to use that should maintain a majority of protein functions.

[Pangea]

No of course inhibition will be happing on the protein level. I mean cutting out the gene will be lethal. But thanks for sharing your knowledge.