Posted 25 April 2013 - 01:20 AM
i believe the residues you mentioned inbetween the GST and the MCS are the protease recognisition sites that enable to remove the GST fusion from the rProtien. Most of the GST vectors do hav this and this also is essential in most applications. however, the HIS tag vector also come but not always with such inclusion of residues that enable the removal of tag.
may be if you want to eliminate you just got to select the vector that doesnt have such cleavage sites.
I always had an alternate hypothesis....