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Avoid SDS in thermal lysis: whole blood?

DNA isolation DNA extraction PCR thermal lysis

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#1 chandniu

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Posted 02 January 2013 - 11:43 PM

Is there a way out to reduce frothing by avoiding SDS (or replacing with something that reduces frothing) in the lysis buffer for whole blood. I am trying to do lysis in a microfluidic channel with heating from the bottom and SDS (1%) causes a lot of frothing that becomes uncontrollable. If I remove SDS, it coagulates, Even 0.5% SDS lead to coagulation.

#2 MScMini

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Posted 03 January 2013 - 11:15 PM

Pending on what you want to lysate, but for for PBMC separation I start with PBS (without Mg and Ca) with 2mM EDTA.
Hope you find it helpful!





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