Dear,
For expression of GFP, one used 5'UTR - GFP - SV40 this sequence. What is the role of SV40 sequence in "5'UTR - GFP - SV40"?
Thank you in advance.
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What is the role of SV40 sequence in "5'UTR - GFP - SV40"?
Started by lioiviei14, Jan 02 2013 07:25 PM
1 reply to this topic
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JYaron
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Posted 02 January 2013 - 10:36 PM
lioiviei14, on 02 January 2013 - 07:25 PM, said:
Dear,
For expression of GFP, one used 5'UTR - GFP - SV40 this sequence. What is the role of SV40 sequence in "5'UTR - GFP - SV40"?
Thank you in advance.
For expression of GFP, one used 5'UTR - GFP - SV40 this sequence. What is the role of SV40 sequence in "5'UTR - GFP - SV40"?
Thank you in advance.
It helps to add the polyA tail to the upstream sequence and acts as a transcriptional terminator sequence. Here is a recent paper describing its effects:
http://www.ncbi.nlm....pubmed/22306880
Jordan Yaron, PhD Candidate
The Biodesign Institute at Arizona State University
www.jordanyaron.com
Ask me about cell biology, microscopy, caspase-1-dependent inflammation and cell death or find my writing at TheBioBlog.com!
The Biodesign Institute at Arizona State University
www.jordanyaron.com
Ask me about cell biology, microscopy, caspase-1-dependent inflammation and cell death or find my writing at TheBioBlog.com!
