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promoter question


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#1 lyok

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Posted 02 January 2013 - 01:54 PM

Hallo,

I have been reading some literature and came across the lacpromoter that is inducible with IPTG.

Now I do not get this.

Do they mean the lacpromoter + lac operon ? If this is the case, I do understand it, because the IPTG can interact with the lacI that is bound on the lacoperator.

However if there is only a lacpromoter, then I do not understand the "IPTG inducible" part.

Is it common to refer to a promoter and by this mean both the promoter and operator sequence?

thanks in advance.

#2 Pangea

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Posted 03 January 2013 - 09:29 AM

The Lac Operon can be on the Vector itself or in the Strains Genome. Lac-Promotor is just to help RNA Polymerase to find his way.

#3 lyok

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Posted 03 January 2013 - 12:03 PM

The Lac Operon can be on the Vector itself or in the Strains Genome. Lac-Promotor is just to help RNA Polymerase to find his way.


I understand this, but its not really an answer to my question.

My problem is that often people mention the lac promoter and they state that it is inducible with IPTG , but how can the lacpromoter be inducible by IPTG?
It needs the lac operator for this?
not?




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