Posted 02 January 2013 - 01:54 PM
I have been reading some literature and came across the lacpromoter that is inducible with IPTG.
Now I do not get this.
Do they mean the lacpromoter + lac operon ? If this is the case, I do understand it, because the IPTG can interact with the lacI that is bound on the lacoperator.
However if there is only a lacpromoter, then I do not understand the "IPTG inducible" part.
Is it common to refer to a promoter and by this mean both the promoter and operator sequence?
thanks in advance.
Posted 03 January 2013 - 09:29 AM
Posted 03 January 2013 - 12:03 PM
The Lac Operon can be on the Vector itself or in the Strains Genome. Lac-Promotor is just to help RNA Polymerase to find his way.
I understand this, but its not really an answer to my question.
My problem is that often people mention the lac promoter and they state that it is inducible with IPTG , but how can the lacpromoter be inducible by IPTG?
It needs the lac operator for this?