Cell attachement occuring after 48 hours, not 24 hours.
Posted 01 January 2013 - 02:45 PM
I was told that the primary cells went through a rough time during their isolation. They were treated with water instead of pbs by mistake and the sorted cells took a long time to grow.
- jeyijpz likes this
Posted 02 January 2013 - 07:03 AM
Posted 02 January 2013 - 10:10 AM
do you still have cells floating after 48 hours? or the ones that attached continued to divide?
After 48 hours most of the cells are attached, there are still a couple of cells floating but not as many as before.
After 72 hours, 99.9% of the cells are attached with barely any floaters.
Posted 02 January 2013 - 01:49 PM
The standard ways to test for mycoplasma contamination are: staining with Hoescht 33258 and looking for extranuclear or extra cellular blue dots (quite small) with a fluorescence microscope, and growing in a soft agar assay. You will not readily see mucoplasma with an ordinary light microscope unless you have a very heavy contamination and some luck. They do not look like ordinary bacteria as they don't have a cell wall and are largely intracellular.