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mean fluorescence intensity, ratio


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#1 leyshmanioz

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Posted 31 December 2012 - 08:11 PM

I am confused in flow data analysis, Can someone explain me what ratio of MFI show us. meaning GFP/RFP ratio.
Thanks

#2 Tabaluga

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Posted 01 January 2013 - 03:58 PM

I am confused in flow data analysis, Can someone explain me what ratio of MFI show us. meaning GFP/RFP ratio.
Thanks


can you give us some more information, such as what did you tag with GFP or RFP ?
Generally speaking, you can compare levels of expression with the MFI ratio.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#3 leyshmanioz

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Posted 01 January 2013 - 08:16 PM

The cells express both GFP and RFP, but RFP has to be silenced, since we see double positive population we look at MFI to see the tendency. I'm not sure how can this ratio be interpreted




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