GST- tagged protein expressed in insoluble
Posted 31 December 2012 - 02:06 AM
I am trying to purify a GST-tagged protein. My protein size is around 37 kDa. After IPTG induction, most of the protein expressed in insoluble. how can i get soluble protein? I already tried different temperatures ( 18,25,30 and 37 C) with different induction time(2hr, 3hr and overnight) and different IPTG concentration (0.1, 0.5, 1mM) and different sonication time(10 sec, 20sec, 30 sec, 40 sec 4cycles). I request you to please help me.Looking for your reply.
Thanks in Advance...
Posted 02 January 2013 - 09:10 AM
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Posted 04 March 2013 - 08:35 PM