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Culture of Drosophila S2R+ cells

S2R+ culture drosophila

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#1 Dai Le

Dai Le

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Posted 29 December 2012 - 07:12 AM

Hi guys,
I'm new to this forum. Lately, I've been working with S2R+ cells and this thing is really pissing me off. I don't know why I cannot culture this thing. I have tried different FBS, both heat inactivated and non heat inactivated. These cells don't seem to attach to the culture surface when I cultured under Schneider + 10%FBS. When I switched to serum free media Sf900 II, they attached to the surface but didn't proliferate. Could anyone with experience in this thing give me some tips?
Thank you guys in advance





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