Hi guys,
I'm new to this forum. Lately, I've been working with S2R+ cells and this thing is really pissing me off. I don't know why I cannot culture this thing. I have tried different FBS, both heat inactivated and non heat inactivated. These cells don't seem to attach to the culture surface when I cultured under Schneider + 10%FBS. When I switched to serum free media Sf900 II, they attached to the surface but didn't proliferate. Could anyone with experience in this thing give me some tips?
Thank you guys in advance
0
No replies to this topic
Also tagged with one or more of these keywords: S2R+, culture, drosophila
Protocols and Techniques Forums →
Animal and Zoology →
Is there anyone who's working with Callosobruchus Maculatus?Started by Guest_Honghu_* , 21 Mar 2013  Callasobruchus, Culture and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
Staining lysed cells in DrosophilaStarted by Guest_levuccio_* , 14 Feb 2013 lysed, drosophila, phage, virus
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Using Labtek for Bone marrow macrophagesStarted by Guest_sentinel01ex_* , 11 Dec 2012 macrophage, labtek, adhersion and 2 more...
|
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
Re-culturing empty plasmidStarted by Guest_Ruth Grace_* , 10 Dec 2012 empty plasmid, culture, ecoli
|
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
Storing Bacterial CultureStarted by Guest_iLiketoTae_* , 05 Dec 2012 bacteria, culture, storage
|
|
|
