I have a few of questions
1)How far off can a polyA signal be placed after the stop codon; i.e what is the optimal range, very close or far off?
2)How far off after the polyA signal can one place a new MCS?
3)Are there major specific differences between bGH or SV40 polya signals regarding different proteins? and which ones are preferred to be placed after something like puromycin?
If you know any good read regarding this topic please let me know.
Where to place the polyA signal?
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