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different PARTS different DNA ,,,


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#1 Hamidrad

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Posted 28 December 2012 - 11:53 AM

Hello all,
I am a food technologist and I am trying to extract dna from pulp and peel of apple, banana and orange. but I want to know, if after dna extraction I am not in trouble to pcr them. I mean in these fruit parts, we have different ploidity... (ex. peel is diploid and pulp is triploid) ??

Edited by Hamidrad, 28 December 2012 - 11:55 AM.


#2 Inbox

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Posted 28 December 2012 - 12:26 PM

relative sequences of DNA in diploid, triploid should be more or less conserved. As long as your primer binding site is not mutated in other chromosome set there should not be problem.

#3 bob1

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Posted 28 December 2012 - 06:40 PM

For straight amplification off the DNA the ploidy should not matter, in fact, the more copies there are, the easier the amplification should be.

#4 Hamidrad

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Posted 29 December 2012 - 11:55 AM

relative sequences of DNA in diploid, triploid should be more or less conserved. As long as your primer binding site is not mutated in other chromosome set there should not be problem.


Thanks,
but you mean If the part which dna extracted from that, was in triploid form, may be mutated after extraction? It may occure because of contaminations?

#5 Inbox

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Posted 30 December 2012 - 12:07 AM

No, I did not mean mutated after extraction/contamination. What I mean is allelic variation in diploid or triploid chromosome set.




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