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siRNA resuspension in RNase-free water - siRNA, microRNA and RNAi - BioForum

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siRNA resuspension in RNase-free water

siRNA resuspension RNase-free water RNAi siRNA buffer

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#1 Neanderthal

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Posted 27 December 2012 - 11:01 PM

Hello,

Recently, we ordered Dharmacon ON-TARGET Plus siRNAs in our lab. We have around 5nmol of siRNAs and we want to make a 100uM stock.

I wonder whether we can use RNase-and DNase-free water to make these concentrated stocks (i.e 100uM) instead of 1x siRNA buffer that the company recommends.

Awaiting your valuable suggestions and comments.

Regards,

Kart

#2 bob1

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Posted 28 December 2012 - 12:28 AM

Invitrogen's siRNAs come with DEPC treated water for reusupension, so I guess the answer is yes.





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