Hello,
Recently, we ordered Dharmacon ON-TARGET Plus siRNAs in our lab. We have around 5nmol of siRNAs and we want to make a 100uM stock.
I wonder whether we can use RNase-and DNase-free water to make these concentrated stocks (i.e 100uM) instead of 1x siRNA buffer that the company recommends.
Awaiting your valuable suggestions and comments.
Regards,
Kart
siRNA resuspension in RNase-free water
Started by Neanderthal, Dec 27 2012 11:01 PM
siRNA resuspension RNase-free water RNAi siRNA buffer
1 reply to this topic
Also tagged with one or more of these keywords: siRNA resuspension, RNase-free water, RNAi, siRNA buffer
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