Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Visualizing RNA on blot


  • Please log in to reply
1 reply to this topic

#1 Ahrenhase

Ahrenhase

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 166 posts
10
Good

Posted 22 December 2012 - 01:17 PM

If the gel is stained with SYBR green, will this transfer (via capillary transfer) onto the blot? When crosslinking a recently transfered blot with UV, I could definitely detect rRNA; however, when I tried to image on a typhoon using SYBR green settings, I was not able to detect anything.

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,674 posts
396
Excellent

Posted 23 December 2012 - 11:49 AM

It should transfer, but it will probably diffuse straight through the blot and get progressively washed out of the RNA.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.