Visualizing RNA on blot
1 reply to this topic
Posted 22 December 2012 - 01:17 PM
If the gel is stained with SYBR green, will this transfer (via capillary transfer) onto the blot? When crosslinking a recently transfered blot with UV, I could definitely detect rRNA; however, when I tried to image on a typhoon using SYBR green settings, I was not able to detect anything.
Posted 23 December 2012 - 11:49 AM
It should transfer, but it will probably diffuse straight through the blot and get progressively washed out of the RNA.