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Visualizing RNA on blot

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#1 Ahrenhase



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Posted 22 December 2012 - 01:17 PM

If the gel is stained with SYBR green, will this transfer (via capillary transfer) onto the blot? When crosslinking a recently transfered blot with UV, I could definitely detect rRNA; however, when I tried to image on a typhoon using SYBR green settings, I was not able to detect anything.

#2 bob1


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Posted 23 December 2012 - 11:49 AM

It should transfer, but it will probably diffuse straight through the blot and get progressively washed out of the RNA.

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