3 replies to this topic

[lioiviei14]

I have 3' biotinylated oligo, Can it be ligated to 5' end of oligo? On what site of DNA a biotin linker is covalently connected?

[phage434]

A 3' biotin will block ligation.  But a 3' biotin-dT base could potentially be ligated.  I don't know how efficient this would be, or if standard ligases would work.

[neuron]

3'-biotin will block OH thats why ligation is not possible. here are some links, which might be helpful-

https://eu.idtdna.co...gold-particles-

http://www.piercenet...?fldID=06010705

phage how is biotin-dT base different? ..

Edited by neuron, 26 December 2012 - 02:56 AM.

[phage434]

Biotin-dT links the biotin to the thymine base, rather than to the 3' -OH group on the ribose.  This leaves the 3' -OH available for linkage to the next base.  Steric issues may make ligase not work or be inefficient, but the block is no longer an issue.