PCR product biotinylation
Posted 19 December 2012 - 06:07 AM
Have anybody done PCR product biotinylation? Can anybody share protocols of doing it? I am trying to uderstand this article http://www.sciencedi...890850804001008 , particulary why they use biotinylated Forward primers, if you can do it just by incorporating already biotinylated dUTP while PCR for example. I would be really grateful if you share some thoughts or info.
Posted 19 December 2012 - 02:23 PM
Posted 20 December 2012 - 12:08 AM