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Genomic DNA extraction with SDS/LiOAc - Molecular Cloning - BioForum

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Genomic DNA extraction with SDS/LiOAc

gDNA pcr SDS/LiOAc

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#1 Zanetti

Zanetti

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Posted 18 December 2012 - 01:41 PM

Hi,


I'm trying to amplify a gene from yeast genomic DNA extracted with the SDS/LiOAc protocol (http://www.biotechni...ues-315236.html).
The extraction is "working" (i have got a quite low concentration, about 35ng/ul) and any of the PCRs i've tried worked.

Anyone already used this protocol? Any suggestions would be appreciated.





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