Hi, I want to observe some small proteins (MW=15 to 25kD) on SDS-PAGE. These are multipule proteins. Do you have recommendations how I can make them stains better, or seperate better to observe?
Thanks a lot!
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#2
bob1
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Thelymitra pulchella
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Posted 18 December 2012 - 11:37 AM
High percent gel is the primary thing you should use. Long gels will help with the separation too.
#3
mdfenko
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Posted 19 December 2012 - 11:56 AM
you can use a tris-tricine sds gel. it gives great resolution of low molecular weight proteins.
you can use colloidal coomassie to get a more intense stain. you can silver stain (it's 50-100x more sensitive than coomassie).
you can use colloidal coomassie to get a more intense stain. you can silver stain (it's 50-100x more sensitive than coomassie).
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