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small proteins


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2 replies to this topic

#1 joy123

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Posted 18 December 2012 - 08:29 AM

Hi, I want to observe some small proteins (MW=15 to 25kD) on SDS-PAGE. These are multipule proteins. Do you have recommendations how I can make them stains better, or seperate better to observe?

Thanks a lot!

#2 bob1

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Posted 18 December 2012 - 11:37 AM

High percent gel is the primary thing you should use. Long gels will help with the separation too.

#3 mdfenko

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Posted 19 December 2012 - 11:56 AM

you can use a tris-tricine sds gel. it gives great resolution of low molecular weight proteins.

you can use colloidal coomassie to get a more intense stain. you can silver stain (it's 50-100x more sensitive than coomassie).
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