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protein Protein Linking

protein bindings

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5 replies to this topic

#1 Gunalan

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Posted 17 December 2012 - 06:44 AM

Hi
Is there any protocol or method to link or bind two different recombinant proteins togather......?........ other than going for cloning them again.....

#2 bob1

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Posted 17 December 2012 - 11:31 AM

Bind two separate proteins physically or express them sequentially (e.g. GFP tagging protein)?

#3 Gunalan

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Posted 17 December 2012 - 09:20 PM

yeah binding them physically

#4 bob1

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Posted 17 December 2012 - 11:47 PM

Sure, check out N-Hydroxysuccinimide-ester.

#5 neuron

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Posted 20 January 2013 - 09:12 PM

If two proteins are linked with NHS, how do we confirm that? I mean control where we don't add NHS will also give two bands of proteins as the linked protein will give??? or is it that linked proteins will show different mobility on native PAGE?

#6 bob1

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Posted 20 January 2013 - 11:56 PM

SHould be different mobility on native PAGE.




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