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how can I remove the pigments during DNA extraction?


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#1 azadeh12345

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Posted 14 December 2012 - 06:32 AM

hi
I want to extract the DNA of a biotrophic fungi from the host tissue, this fungus causes the tissue turn to red.
After DNA extraction I have red pigments in my total DNA. also I have low PCR product, which I think is due to some inhibitors (probably the pigmets). how can I remove them?
Thank you.

#2 bob1

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Posted 14 December 2012 - 07:20 PM

Which of the many DNA extraction protocols are you using? How does the DNA look on a gel and a spec?

Have you tried diluting your template for the PCR? How big a product are you generating? Is the sequence GC rich? Any other details you can think of that might help...




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