Immunoprecipitated protein visible on coomassie but not detected by Western
Posted 14 December 2012 - 03:42 AM
I am immunoprecipitating my protein with its antibody. I run the eluted proteins onto the gel and could see the immunoprecipited bans on coomassie. But I could not detect the protein by western analysis. What could be the possible explanation for this bizzare phenomena? Please help me. Lots of thanks in advance.
Posted 14 December 2012 - 07:06 AM
that your antibody is in good condition and usable for western blot (check the data sheet and run a positive control)?
genius does what it must
i do what i get paid to do
Posted 14 December 2012 - 07:28 AM