I imaged Cy3 gels using the Typhoon scanner coupled with a proper set of Laser, Excitation and Emission filters.
Yet, in addition to the conventional 'dark' Cy3 bands, I also observed that certain protein bands appeared as 'white' on the same gel.
Have you encountered this before? Or what may be the possible cause of this?
P.S. I have imaged the same gel using another fluorescent scanner (Pharos FX; Bio-rad). The 'white' bands still persisted, suggesting that it was likely not the scanner problem.
Thanks a lot in advance! Please kindly give me some advise.
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White bands on Cy3 gels
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