I wanna run a monooxygenase titration assay and measure the content of haem. However, i dont have a microplate reader with 650 nm wavelength (the highest i have is 595nm). Is it alright if i use a regular spectrophotometer? Will there be any concern on the data obtained if i do so?
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#2
prabhubct
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Posted 11 December 2012 - 04:55 AM
I guess you can use spectrophotometer for it.
See http://www.protocol-...de3-ripl-cells/
Ascacioc post #7
But how many samples you will be doing and time gap you need to think of.
See http://www.protocol-...de3-ripl-cells/
Ascacioc post #7
But how many samples you will be doing and time gap you need to think of.
Edited by prabhubct, 11 December 2012 - 05:02 AM.
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#3
bob1
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Posted 11 December 2012 - 11:32 AM
So long as you aren't measuring fluorescence of the haem, then you should be fine.
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hianghao
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Posted 12 December 2012 - 04:30 AM
prabhubct, on 11 December 2012 - 04:55 AM, said:
I guess you can use spectrophotometer for it.
See http://www.protocol-...de3-ripl-cells/
Ascacioc post #7
But how many samples you will be doing and time gap you need to think of.
See http://www.protocol-...de3-ripl-cells/
Ascacioc post #7
But how many samples you will be doing and time gap you need to think of.
bob1, on 11 December 2012 - 11:32 AM, said:
So long as you aren't measuring fluorescence of the haem, then you should be fine.
Ya, the more important part is the time frame. Thank you for the advises!
