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Can i use spectrophotometer to replace microplate reader


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#1 hianghao

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Posted 11 December 2012 - 02:19 AM

I wanna run a monooxygenase titration assay and measure the content of haem. However, i dont have a microplate reader with 650 nm wavelength (the highest i have is 595nm). Is it alright if i use a regular spectrophotometer? Will there be any concern on the data obtained if i do so?

#2 Inbox

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Posted 11 December 2012 - 04:55 AM

I guess you can use spectrophotometer for it.

See http://www.protocol-...de3-ripl-cells/
Ascacioc post #7


But how many samples you will be doing and time gap you need to think of.

Edited by prabhubct, 11 December 2012 - 05:02 AM.


#3 bob1

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Posted 11 December 2012 - 11:32 AM

So long as you aren't measuring fluorescence of the haem, then you should be fine.

#4 hianghao

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Posted 12 December 2012 - 04:30 AM

I guess you can use spectrophotometer for it.

See http://www.protocol-...de3-ripl-cells/
Ascacioc post #7


But how many samples you will be doing and time gap you need to think of.

So long as you aren't measuring fluorescence of the haem, then you should be fine.


Ya, the more important part is the time frame. Thank you for the advises!




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