Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Re-culturing empty plasmid

empty plasmid culture ecoli

  • Please log in to reply
1 reply to this topic

#1 Ruth Grace

Ruth Grace

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 10 December 2012 - 11:50 PM

Greetings Posted Image

I am new in molecular biology lab. I was given an ecoli + empty pgsa plasmid and my sv ask me to reculture them.

My questions are:

1) What is an empty plasmid?
2) When I reculture them, my sv ask me to add antibiotics. What is the purpose of doing that?

Thank you for all the brilliant answers! :)

#2 christy

christy

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 40 posts
2
Neutral

Posted 11 December 2012 - 12:02 AM

Empty plasmid is just circular plasmid, without our interest gene. Check the antibiotic of the plasmid. For example, pGEMT vector is carrying ampicilin resistance gene. To cultivate this plasmid, you have to add 50 or 100 ug/ml ampicillin in the medium for plasmid transformed E. coli. Otherwise, the plasmid will not replicate. To cultivate the E. coli with plasmid we have to add appropriate anitibiotic in the medium.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.