Hey guys, just wondering if anyone has a buffer/enzyme mix they use to reduce cell death when generating a single cell suspension from neuronal tissue.
I'm looking to run some flow cytometry with them after isolation.
Thanks, Jacob
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Generation of single cell suspensions from neural tissue
Started by JacobThomas, Dec 10 2012 08:35 PM
Neurons neuronal single cell enzymes
2 replies to this topic
#3
Tabaluga
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Making glass out of shards
54
Excellent
Excellent
Posted 06 January 2013 - 10:22 PM
Have you tried Accutase ?
Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.
Also tagged with one or more of these keywords: Neurons, neuronal, single cell, enzymes
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