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Generation of single cell suspensions from neural tissue

Neurons neuronal single cell enzymes

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#1 JacobThomas

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Posted 10 December 2012 - 08:35 PM

Hey guys, just wondering if anyone has a buffer/enzyme mix they use to reduce cell death when generating a single cell suspension from neuronal tissue.

I'm looking to run some flow cytometry with them after isolation.

Thanks, Jacob

#2 JacobThomas

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Posted 06 January 2013 - 03:26 PM

anyone?

#3 Tabaluga

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Posted 06 January 2013 - 10:22 PM

Have you tried Accutase ?





Also tagged with one or more of these keywords: Neurons, neuronal, single cell, enzymes

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