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Generation of single cell suspensions from neural tissue

Neurons neuronal single cell enzymes

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2 replies to this topic

#1 JacobThomas

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Posted 10 December 2012 - 08:35 PM

Hey guys, just wondering if anyone has a buffer/enzyme mix they use to reduce cell death when generating a single cell suspension from neuronal tissue.

I'm looking to run some flow cytometry with them after isolation.

Thanks, Jacob

#2 JacobThomas

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Posted 06 January 2013 - 03:26 PM

anyone?

#3 Tabaluga

Tabaluga

    Making glass out of shards

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Posted 06 January 2013 - 10:22 PM

Have you tried Accutase ?

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 






Also tagged with one or more of these keywords: Neurons, neuronal, single cell, enzymes

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