Hey guys, just wondering if anyone has a buffer/enzyme mix they use to reduce cell death when generating a single cell suspension from neuronal tissue.
I'm looking to run some flow cytometry with them after isolation.
Thanks, Jacob
Submit your paper to J Biol Methods today!
Generation of single cell suspensions from neural tissue
Started by JacobThomas, Dec 10 2012 08:35 PM
Neurons neuronal single cell enzymes
2 replies to this topic
#3
Tabaluga
-
- Active Members
-
- 293 posts
And we're back to square one
39
Excellent
Excellent
Posted 06 January 2013 - 10:22 PM
Have you tried Accutase ?
Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.
Also tagged with one or more of these keywords: Neurons, neuronal, single cell, enzymes
Protocols and Techniques Forums →
Cell Biology →
Magnet assisted transfectionStarted by Alina87, 25 Jun 2013  magnet, transfection and 3 more...
|
|
|
||
Protocols and Techniques Forums →
Molecular Cloning →
What is NEB's CutSmart Buffer?Started by jerryshelly1, 26 Mar 2013 molecular biology, enzymes and 3 more...
|
|
|
||
Protocols and Techniques Forums →
Protein Expression and Purification →
How to improve enzyme activity?Started by aodonnell311, 19 Mar 2013 enzymes, assays, substrates and 2 more...
|
|
|
||
Protocols and Techniques Forums →
Neuroscience →
Primary Neuron ContaminationStarted by Mighty Mouse, 24 Apr 2012 Neurons, Primary culture and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Stem Cell →
differentiating neurons detaching from plateStarted by jlolsen, 09 Apr 2012 stem cells, neurons and 1 more...
|
|
|
