Vibratome (~ 150um thick slice) to identify GFP signal in the tissues
Posted 08 December 2012 - 12:04 PM
I injected GFP expressed cells into the mouse and i would like to identify where the GFP cells will be distributed/circulated in the normal mouse.
I would like to ask if I can use the vibratome (~ 150um thick slice) to identify GFP signal in the organs (lungs, heart, liver, spleen , pancreas, brain) which are fixed in 4% parafolmadehye?
Or i can PCR the organs to see if there's band for the GFP.
Which way is better to identify/detect the GFP cells or any other suggestions?
Posted 08 December 2012 - 12:21 PM
Either method will work well. However, the PCR will only detect the presence of DNA, unless you do some reverse transcription-PCR to detect transcript, the presence of DNA doesn't indicate that GFP would be expressed there. YOu could also do western blots on the tissues.
Posted 10 December 2012 - 03:21 AM
Thank you very much for the information and the suggestions.
You have been a great help
Have a nice day.