I injected GFP expressed cells into the mouse and i would like to identify where the GFP cells will be distributed/circulated in the normal mouse.
I would like to ask if I can use the vibratome (~ 150um thick slice) to identify GFP signal in the organs (lungs, heart, liver, spleen , pancreas, brain) which are fixed in 4% parafolmadehye?
Or i can PCR the organs to see if there's band for the GFP.
Which way is better to identify/detect the GFP cells or any other suggestions?
Thank you.

wdyeo