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microRNA promoter based expression vector?

microRNA expression

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#1 assembler01

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Posted 07 December 2012 - 12:03 PM

Is it possible to design a transgene to express a marker protein under the control of a miR promoter (when the miR is expressed from it's own non-coding piece of RNA, obviously not when it's expressed intragenically)? For example, can you have GFP or betaGal expressed under the control of a pre-miR ncRNA to see where and when the pre-miR is expressed? Has anyone done that before? I know you can check for the RNA itself by reverse transcription-PCR or something, or even check for the miR with a luciferase assay, but I was wondering about expressing something with the miR promoter.

Thanks.

#2 pcrman

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Posted 07 December 2012 - 05:44 PM

Actually most microRNAs are transcribed from Pol II promoter even they are independently transcribed from protein coding gene. many companies sell GFT-linked miRNA expression vectors under the control of a Pol II promoter.





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