Large scale Viral RNA purification Kit
Posted 06 December 2012 - 10:21 AM
I Infected hela cells with virus, and left it for 24 hours in 100mm dish ( 10 ml media) . Now i separated the cells which 99% of them were killed off by the virus, and took the supernatant. From this supernatant which is 10ml, i want to isolate the viral RNA. QIAGEN kits can deal with small amount of samples. Here , i have got 10ml.
What would you advice me to do? what is the best kit or/and method around to deal with this issue?
Posted 06 December 2012 - 05:56 PM
Posted 07 December 2012 - 05:23 AM
You are absolutely right. But i am looking for a specific Recombination event, so i need to isolate every thing in the media.
Posted 07 December 2012 - 07:29 PM