1 reply to this topic

[iLiketoTae]

I have my recombinant bacterial sample stored on agar plates. I didn't realize that condensed water had built up on the bottom, so when I flipped it, some of the water mixed with my colonies. Are they still good? Should I restreak them? And for restreaking, do I grow them in a liquid culture then plate them? or can I streak straight from a colony?

Edited by iLiketoTae, 05 December 2012 - 04:56 AM.

[pito]

iLiketoTae, on 05 December 2012 - 04:54 AM, said:

I have my recombinant bacterial sample stored on agar plates. I didn't realize that condensed water had built up on the bottom, so when I flipped it, some of the water mixed with my colonies. Are they still good? Should I restreak them? And for restreaking, do I grow them in a liquid culture then plate them? or can I streak straight from a colony?

The water itself is from the plates, so there should not really be any contamination.
But you do need to restreak them anyway, you cant store them for too long like that.
(you cant "store" your samples like that... you can keep them for a while , but not "store" them, if you know what I mean...)
To really store them you need to make a glycerolstock for example/

And just restreak them on a new plate. Pick a colony, restreak it. Best you pick a few colonies, streak a few colonies on a few plates.
(for 1 plate, 1 colony)