GFP analysis and lab help
Posted 05 December 2012 - 02:44 AM
How could you quantitate the average proportion of total GFP that is localised to the nucleus per cell
How could a cell maintain a cytoplasmically localised transcription factor such that after a stimulus this factor would relocate to the nucleus?
How could you use recombinant Ran protein to purify multiple nuclear import factors and iclude two control incubations to ensure the factors isolated are specific Ran binding partners?
Posted 05 December 2012 - 01:54 PM
Posted 05 December 2012 - 02:45 PM