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SDS-PAGE related to sample containing chloroform


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#1 ira

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Posted 05 December 2012 - 12:13 AM

Hi everyone,

I would like to ask if my sample was added with a little amount of chloroform (well-known that chloroform built from chloride ion), is it the chloroform will affect the result of sds?

#2 neuron

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Posted 20 December 2012 - 01:46 AM

I think yes it would interfere with your results, once I tired running the samples that contained ethyl acetate, I did not get any bands. Probably because solvents are lighter then water and also they don't mix with bufferPosted Image

Edited by neuron, 20 December 2012 - 01:47 AM.

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#3 ira

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Posted 25 February 2013 - 05:48 AM

owh..tq so much! :)




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