Does anyone knows a well established protocol for cytoplasmic mRNA extraction without using Phenol:chloroform:Isoamylalcohol or a kit? I am doing qPCR afterwards, that is why I do not want to use a phenol-based protocol (and I do not have a kit neither).
Thank you
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Cytoplasmic mRNA isolation protocol
Started by Niwrad, Dec 04 2012 12:08 AM
cytoplasmic mRNA protocol
1 reply to this topic
#2
bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 04 December 2012 - 11:26 AM
Phenol:chloroform extractions are still the gold standard for these sorts of things as far as I am aware. I don't think there are many options besides kits and P:C.
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