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Cytoplasmic mRNA isolation protocol

cytoplasmic mRNA protocol

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#1 Niwrad

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Posted 04 December 2012 - 12:08 AM

Does anyone knows a well established protocol for cytoplasmic mRNA extraction without using Phenol:chloroform:Isoamylalcohol or a kit? I am doing qPCR afterwards, that is why I do not want to use a phenol-based protocol (and I do not have a kit neither).

Thank you

#2 bob1

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Posted 04 December 2012 - 11:26 AM

Phenol:chloroform extractions are still the gold standard for these sorts of things as far as I am aware. I don't think there are many options besides kits and P:C.





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